Uses[ edit ] Affinity chromatography can be used to purify and concentrate a substance from a mixture into a buffering solution, reduce the amount of unwanted substances in a mixture, identify the biological compounds binding to a particular substance, purify and concentrate an enzyme solution.
Drawing markers not permanent: Cut at least two strips, one to test brown and one to test yellow. Cut an extra strip for each additional color you would like to test. How do you expect each of the different colors to behave when you test it with the paper strip?
Draw a pencil line across the width of each paper strip, about one centimeter from the bottom end. Take the brown marker and a paper strip and draw a short line about one centimeter on the middle section of the pencil line.
Your marker line should not touch the sides of your strip. Use a pencil to write the color of the marker you just used on the top end of the strip.
Do not use the colored marker or pen to write on the strips, as the color or ink will run during the test. Repeat the previous three steps with a yellow marker and then all the additional colors you would like to test.
Hold a paper strip next to one of the tall glasses on the outside of italigning the top of the strip with the rim of the glass, then slowly add water to the glass until the level just reaches the bottom end of the paper strip.
Repeat with the other glass eskeeping the strips still on the outside and away from the water. What role do you think the water will play?
Procedure Fasten the top of a strip the side farthest from the marker line to the pencil with a binder clip or clothespin. Pause for a moment. Do you expect this color to be the result of a mixture of colors or the result of one color molecule?
If you like, you can make a note of your prediction now. Hang the strip in one of the glasses that is partially filled with water by letting the pencil rest on the glass rim. The bottom end of the strip should just touch the water level. If needed, add water to the glass until it is just touching the paper.
It is important that the water level stays below the marker line on the strip. Leave the first strip in its glass as you repeat the previous two steps with the second strip and the second glass.
Repeat with any additional colors you are testing. Watch as the water rises up the strips. What happens to the colored lines on the strips?
Does the color run up as well? Do you see any color separation? When the water level reaches about one centimeter from the top this may take up to 10 minutesremove the pencils with the strips attached from the glasses.
If you let the strips run too long, the water can reach the top of the strips and distort your results.
Write down your observations. Did the colors run? Did they separate in different colors? Which colors can you detect?Chromatography is used to separate mixtures of substances into their components.
All forms of chromatography work on the same principle. They all have a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas).
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93 7. Thin-Layer Chromatography You will need to bring your package of TLC plates on this day.
Not all plates will be used in this experiment; some will be needed in lab the following week. Where Science Meets Service. Alfa Aesar is a leading manufacturer and supplier of research chemicals, metals and materials for a wide span of applications.
Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition.
93 7. Thin-Layer Chromatography You will need to bring your package of TLC plates on this day. Not all plates will be used in this experiment; some will be needed in lab the following week.